Transient Posttranscriptional Gene Silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS).

Joanna Serwatowska,Ole Søgaard Lund,Ida Elisabeth Johansen

FUNCTIONAL GENOMICS IN MEDICAGO TRUNCATULA: METHODS AND PROTOCOLS（2018）

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摘要

Successful application of virus-induced gene silencing for functional genomics requires a virus vector that can initiate a systemic infection of the host plant. Agroinoculation of the pea early browning virus vectors pCAPE1 and pCAPE2 can establish infection in several genotypes of Medicago truncatula and can reduce target gene RNA levels to an extent that allows investigation of gene function.

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关键词

Functional genomics,PEBV,Pea early browning virus,Reverse genetics,Virus vector

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