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The Inhibitory Effect of Mouse Gastric DNA on Amplification of Helicobacter pylori Genomic DNA in Quantitative PCR

Mehdi Alikhani,Ebrahim Shafaie,Esmat Mirabzadeh Ardakani,Maryam Esmaeili,Samaneh Saberi,Mojgan Hatefi,Marjan Mohammadi

Iranian biomedical journal（2019）

Cited 1|Views6
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Abstract
Our method, bypassing the inhibition and/or interference imposed by MgDNA, was able to quantitate gastric tissue-colonizing Hp, segregating mice inoculated with low vs. high doses of Hp, as well as those immunized from controls.
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Key words
Amplification,Inhibition,Interference,Standard curve,Quantitative PCR
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