Effects of Cyclic Tensile Strain on Oxidative Stress and the Function of Schwann Cells.

Schwann cells (SCs) are significant due to the way in which they sustain and myelinate axons within the peripheral nervous system (PNS). This study has investigated the effect of cyclic tensile strain (CTS) on the oxidative stress and function of SCs derived from the sciatic nerves of an infant rat population. A group of 20 6-day-old Wistar rats was selected, and SCs were separated from the sciatic nerve. The SCs then underwent a 6-hour period of cyclical straining, and ElectroForce 3200 in combination with the BioDynamic chamber was employed to apply 0% and 5% strains at a 0.25 Hz frequency. The results showed that the control group suffered higher oxidative stress than that in 5% strains group (P<0.05). The results RT-PCR analysis indicated a correlation between 5% CTS and a reduction in Netrin-1 expression (P<0.05). Furthermore, there was a significant upregulation in NGF, GDNF, and Slit-2 gene expression (P<0.05). Finally, the results showed that CTS stimulate SCs by increasing the expression of nerve-oriented factors, and these importantly caused the decrease of oxidative stress, reconstruction of cell skeleton, the promotion of axonal regrowth, and the augmentation of nerves.