Resistance of mouse primary microglia and astrocytes to acrylonitrile-induced oxidative stress.

•We addressed glial cell viability, ACN uptake, glutathione (GSH) levels and the expression of NF-E2-related factor 2 (Nrf2) in response to ACN treatment.•Mouse glial cells were resistant to ACN-induced oxidative stress.•Both cell types accumulated ACN; however, there was a minor effect of ACN on cell viability in astrocytes and microglia.•Nrf2 and GSH levels were unchanged in ACN-treated as compared to the untreated cells.•These observations suggest that primary mouse glial cells are resistant to ACN.