Genetic Abrogation Of The Fibronectin-Alpha 5 Beta 1 Integrin Interaction In Articular Cartilage Aggravates Osteoarthritis In Mice

The balance between synthesis and degradation of the cartilage extracellular matrix is severely altered in osteoarthritis, where degradation predominates. One reason for this imbalance is believed to be due to the ligation of the alpha 5 beta 1 integrin, the classic fibronectin (FN) receptor, with soluble FN fragments instead of insoluble FN fibrils, which induces matrix metalloproteinase (MMP) expression. Our objective was to determine whether the lack of alpha 5 beta 1-FN binding influences cartilage morphogenesis in vivo and whether non-ligated alpha 5 beta 1 protects or aggravates the course of osteoarthritis in mice. We engineered mice (Col2a-Cre;Fn1(RGE/fl)), whose chondrocytes express an alpha 5 beta 1 binding-deficient FN, by substituting the aspartic acid of the RGD cell-binding motif with a glutamic acid (FN-RGE). At an age of 5 months the knee joints were stressed either by forced exercise (moderate mechanical load) or by partially resecting the meniscus followed by forced exercise (high mechanical load). Sections of femoral articular knees were analysed by Safranin-O staining and by immunofluorescence to determine tissue morphology, extracellular matrix proteins and matrix metalloproteinase expression. The articular cartilage from untrained control and Col2a-Cre;Fn1(RGE/fl) mice was normal, while the exposure to high mechanical load induced osteoarthritis characterized by proteoglycan and collagen type ll loss. In the Col2a-Cre;Fn1(RGE/fl) articular cartilage osteoarthritis progressed significantly faster than in wild type mice. Mechanistically, we observed increased expression of MMP-13 and MMP-3 metalloproteinases in FN-RGE expressing articular cartilage, which severely affected matrix remodelling. Our results underscore the critical role of FN-alpha 5 beta 1 adhesion as ECM sensor in circumstances of articular cartilage regeneration.