Langerin + CD8α + Dendritic Cells Drive Early CD8 + T Cell Activation and IL-12 Production During Systemic Bacterial Infection.

Bloodstream infections induce considerable morbidity, high mortality, and represent a significant burden of cost in health care; however, our understanding of the immune response to bacteremia is incomplete. Langerin(+) CD alpha(+) dendritic cells (DCs), residing in the marginal zone of the murine spleen, have the capacity to cross-prime CD8(+) T cells and produce IL-12, both of which are important components of antimicrobial immunity. Accordingly, we hypothesized that this DC subset may be a key promoter of adaptive immune responses to blood-borne bacterial infections. Utilizing mice that express the diphtheria toxin receptor under control of the langerin promoter, we investigated the impact of depleting langerin(+) CD8 alpha(+) DCs in a murine model of intravenous infection with Mycobacterium bovis bacille Calmette-Guerin (BCG). In the absence of langerin(+) CD8 alpha(+) DCs, the immune response to blood-borne BCG infection was diminished: bacterial numbers in the spleen increased, serum IL-12p40 decreased, and delayed CD8(+) T cell activation, proliferation, and IFN-gamma production was evident. Our data revealed that langerin(+) CD8 alpha(+) DCs play a pivotal role in initiating CD8(+) T cell responses and IL-12 production in response to bacteremia and may influence the early control of systemic bacterial infections.