Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction.

Charline J J Gerard,Gilles Ferry,Laurent M Vuillard,Jean A Boutin,Leonard M G Chavas,Tiphaine Huet,Nathalie Ferte,Romain Grossier,Nadine Candoni,Stéphane Veesler

Acta crystallographica. Section F, Structural biology communications（2017）

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摘要

A microfluidic platform was used to address the problems of obtaining diffraction-quality crystals and crystal handling during transfer to the X-ray diffractometer. Crystallization conditions of a protein of pharmaceutical interest were optimized and X-ray data were collected both in situ and ex situ.

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关键词

crystallization,ex situ X-ray diffraction,in situ X-ray diffraction,microfluidics,optimization

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