Sequence-specific sepsis-related DNA capture and fluorescent labeling in monoliths prepared by single-step photopolymerization in microfluidic devices.

•DNA capture monoliths were formed by a single-step process in a microchip.•The monoliths had better characteristics than ones made by a multistep procedure.•Unlabeled DNA related to sepsis was captured, labeled and eluted.•DNA capture was feasible from lysed bacteria enriched from blood samples.