A proteomic approach to identify metalloproteins and metal-binding proteins in liver from diabetic rats

Camila Pereira Braga,José Cavalcante Souza Vieira,Ryan A. Grove,Cory H.T. Boone,Aline de Lima Leite,Marília Afonso Rabelo Buzalaf,Ana Angélica Henrique Fernandes,Jiri Adamec,Pedro de Magalhaes Padilha

International Journal of Biological Macromolecules（2017）

引用 15|浏览7

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摘要

•Metalloproteomic study in liver samples of diabetics rats.•Protein separation by a two-dimensional gel electrophoresis.•Identification of cooper, selenium and zinc present in the protein spots by FAAS or GFASS.•It was possible to characterize 75 protein spots by ESI–MS/MS.

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关键词

Electrospray ionization-tandem mass spectrometry,Flame atomic absorption spectrometry,Graphite furnace atomic absorption spectrometry,Metalloproteomic,Type 1 diabetes,Two-dimensional electrophoresis

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