Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF).
MULTIPLE MYELOMA: METHODS AND PROTOCOLS(2018)
摘要
Mass cytometry has emerged as a new state-of-the-art technology that enables in-depth characterization of cellular populations and functions at a single cell resolution. We describe the application of this technology to deeply phenotype the blood and bone marrow components of multiple myeloma patients in a clinical setting. This technology allows for simultaneous quantification of more than 40 markers, overcoming the challenges of traditional fluorescence-based flow cytometry.
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关键词
Mass cytometry, CyTOF, Multiple myeloma, Immunophenotyping, Single cell, Barcoding, Smart tube, Peripheral blood, Bone marrow
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