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UPLC-Q-TOF-MS/MS快速分析苁蓉总苷胶囊化学成分

吴金凤,尹权微,潘有智,王雪,秦建平,李家春,黄文哲,王振中

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica（2016）

Cited 6|Views14
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Abstract
该文采用超高效液相色谱-四极杆飞行时间高分辨率质谱(UPLC-Q-TOF-MS/MS)技术对苁蓉总苷胶囊中化学成分进行了定性分析.液相条件:Agilent SB-C18Rapid Resolution HD(3.0 mm×100 mm,1.8 μm)色谱柱,以乙腈-0.1％甲酸水溶液为流动相进行梯度洗脱,体积流量0.2 mL·min-1,柱温30℃,检测波长330 nm;质谱条件:采用ESI源,负离子模式下进行数据采集.通过与对照品对比、数据库匹配分析及参考文献资料,共推测出苁蓉总苷胶囊中的41个化合物.该方法能快速检测苁蓉总苷胶囊的化学成分,为苁蓉总苷胶囊的质量控制提供参考,也为其药效物质基础的进一步研究奠定基础.
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Key words
Congrong Zonggan capsule,UPLC-Q-TOF-MS/MS,chemical components,fragmentation pattern,mass spectrum characteristics
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