Establishing anaerobic hydrocarbon-degrading enrichment cultures of microorganisms under strictly anoxic conditions

Rafael Laso-Pérez,Viola Krukenberg,Florin Musat,Gunter Wegener

NATURE PROTOCOLS（2018）

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摘要

Many anaerobic microorganisms are difficult to isolate because they grow slowly and rely on interactions with other microorganisms. This protocol describes an approach for the successive enrichment of syntrophic hydrocarbon-degrading microorganisms.

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关键词

Archaeal physiology,Microbiology techniques,Water microbiology,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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