Phenotyping Tumor-Associated Macrophages.
MACROPHAGES: METHODS AND PROTOCOLS（2018）
Abstract
Multi-parametric flow cytometry of tumor-bearing murine nonlymphoid tissue allows for characterization, isolation, and examination of immune cell composition, phenotype, and function. Here we describe an approach to process nonlymphoid tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This panel can be used to phenotype tumor-associated macrophages.
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Key words
Dendritic cells,Flow cytometry,Granulocytes,Immune cells,Immunophenotyping,Lymphocytes,Macrophages,Monocytes,Mononuclear phagocytes,Tumor-associated macrophages
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