Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing

An Xu,Ruoji Zhou,Jian Tu,Zijun Huo,Dandan Zhu,Donghui Wang,Julian A. Gingold, Helen Mata,Pulivarthi H. Rao,Mo Liu,Alaa M.T. Mohamed,Celine Shuet Lin Kong,Brittany E. Jewell,Weiya Xia,Ruiying Zhao,Mien-Chie Hung,Dung-Fang Lee

Stem Cell Research（2018）

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摘要

Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.

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