TROSY pulse sequence for simultaneous measurement of the 15 N R 1 and { 1 H}– 15 N NOE in deuterated proteins

A TROSY-based NMR experiment is described for simultaneous measurement of the 15 N longitudinal relaxation rate constant R 1 and the { 1 H}– 15 N nuclear Overhauser enhancement. The experiment is based on the observation that the TROSY mixing pulse sequence element symmetrically exchanges 1 H and 15 N magnetizations. The accuracy of the proposed technique is validated by comparison to independent measurements of both relaxation parameters for the protein ubiquitin. The simultaneous experiment is approximately 20–33% shorter than conventional sequential measurements.