Retracted: Signalling Cross-Talk Between Nitric Oxide And Active Oxygen In Trifolium Repens L. Plants Responses To Cadmium Stress (Retracted Article. See Vol. 243, Pg. 1966, 2018)

The significant influence of (NO)-N-center dot on the stress response is well established; however, the precise meta-bolic pathways of (NO)-N-center dot and RNS under metal stresses remain unclear. Here, the key components of ROS and RNS metabolism under Cd stress were investigated with multi-level approaches using high-quality forage white clover (Trifolium repens L) plants. For the studied plants, Cd disturbed the redox homeostasis, affected the absorption of minerals, and exacerbated the degree of lipid peroxidation, thus triggering oxidative stress. However, (NO)-N-center dot was also involved in regulating mineral absorption, ROS-scavenger levels and mRNA expression in Cd-treated white clover plants. In addition, GSNOR activity was up regulated by Cd with the simultaneous depletion of (NO)-N-center dot generation and GSNO but was counteracted by the (NO)-N-center dot donor sodium nitroprusside. Response to Cd-stressed SNOs was involved in generating ONOO- and NO2-Tyr in accordance with the regulation of (NO)-N-center dot-mediated post-translational modifications in the ASC-GSH cycle, selected amino acids and NADPH-generating dehydrogenases, thereby provoking nitrosative stress. Taken together, our data provide comprehensive metabolite evidence that clearly confirms the relationships between ROS and RNS in Cd-stressed plants, supporting their regulatory roles in response to nitro-oxidative stress and providing an in-depth understanding of the interaction between two families subjected to metal stresses. (C) 2018 Elsevier Ltd. All rights reserved.