Chaperonin-Containing T-Complex Protein 1 Subunit 8 Promotes Cell Migration And Invasion In Human Esophageal Squamous Cell Carcinoma By Regulating Alpha-Actin And Beta-Tubulin Expression

The chaperonin-containing T-complex protein 1 (CCT) has eight subunits, CCT 1-8, which are dysregulated in several types of cancer. To determine how subunit 8 (CCT8) influences the development of esophageal squamous cell carcinoma (ESCC), immunohistochemistry and western blot analysis were performed on 128 ESCC samples in the present study to measure the expression of CCT8. The prognostic value of CCT8 was analyzed using univariate and multivariate survival analyses. CCT8 knockdown in ESCC cells was performed and subsequently, the migration and invasion of ESCC cells was assessed. The results of immunohistochemistry and western blot analysis of ESCC tissue indicated that the expression of CCT8 in tumor tissues from patients with lymph node metastasis (LNM) was high whereas its expression in tissues from those without LNM was low. In addition, the overall survival rate of patients with high CCT8 expression was poor. It was demonstrated that CCT8 influenced the migration and invasion of ESCC cells by regulating alpha-actin and beta-tubulin. Following CCT8 knockdown, cells were treated with cisplatin; it was demonstrated that alpha-actin and beta-tubulin were downregulated and that cell apoptosis was enhanced. These data confirm that alpha-actin and beta-tubulin are regulated by CCT8, and that increased CCT8 expression is associated with poor patient prognosis and cisplatin resistance in ESCC.