内生细菌BZJN1的鉴定及对白术根腐病的生物防治研究

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica(2018)

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Abstract
该研究从白术体内分离得到一株细菌BZJN1,经生理生化检测及分子鉴定证明其为枯草芽孢杆菌Bacillus subtilis.菌株BZJN1可以显著抑制白术角担菌菌丝生长,抑制率超过70%.显微观察对峙区域发现角担菌菌丝生长畸形、菌丝顶端或中部膨大呈球形,原生质泄露.其发酵滤液在3%,6%的浓度下对角担菌菌丝生长抑制率达到22.7%,38.7%.田间防效试验证明,药后10 d,BZJN1生防菌剂在1×109 cfu·mL-1处理防效最高,达到了75.27%.药后20 d,1 ×109 cfu·mL-1的处理防效为72.37%,和化学药剂相当.所有BZJN1的处理对白术有一定促生效果,1×109 cfu·mL-1处理,增产达到14.1%.
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Key words
Atractylodes macrocephala,Bacillus subtilis,Ceratobasidium sp,control
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