The pseudo-dimeric tyrosyl-tRNA synthetase of T. brucei aminoacylates cytosolic and mitochondrial tRNA and requires both monomeric units for activity.
Molecular and Biochemical Parasitology（2018）
Abstract
•Tb-TyrRS is essential for normal growth in procyclic T. brucei.•Tb-TyrRS is the only enzyme responsible for charging of both cytosolic and mitochondrial tRNATyr.•Short N- or C-terminal deletions inactivate Tb-TyrRS.
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Key words
Trypanosoma brucei,tRNA,Mitochondria,Aminoacyl-tRNA synthetase
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