Polymerase bypass of N7-guanine monoadducts of cisplatin, diepoxybutane, and epichlorohydrin.

•DNA adducts of cisplatin, diepoxybutane, and epichlorohydrin have relevance in cancer therapy and carcinogenesis.•We investigated the integrity of DNA synthesis from templates containing N7-guanine monoadducts of these agents.•Two bacterial polymerases and human polymerase successfully bypassed the lesions, although with reduced efficiency.•The polymerases did not show significant misincorporation near the lesions.•N7-guanine monoaddducts do not appear to contribute significantly to the mutational spectra of these agents.