Zebrafish as a visual and dynamic model to study the transport of nanosized drug delivery systems across the biological barriers.

•Transport of C6-NCs across biological barriers has been studied in zebrafish model.•Higher transport efficiency was observed with small sized NCs.•Accumulation and excretion of C6-NCs in different organs have been investigated.•Lipid raft active pathways participated the transport of NCs.•Intact C6-NCs together with the free dissolved form of C6 were observed in vivo.