Evaluation of regulatory T lymphocytes and IL2Ra and FOXP3 gene expression in peripheral mononuclear cells from patients with amyotrophic lateral sclerosis
Irish journal of medical science(2018)
Abstract
Background Loss of neuroprotective role of CD4 + helper T cells, regulatory T cells, and M2 microglia constitutively results in the rapid neural death in the “rapidly progressive phase” of amyotrophic lateral sclerosis (ALS). Aim We aimed to investigate relative count of CD4 + and regulatory T lymphocytes and expression level of IL2Ra and FOXP3 genes in peripheral blood mononuclear cells (PBMCs) from patients with ALS. Method We performed a flow cytometric analysis on PBMC from 38 patients with ALS and 32 controls to determine the count of CD4 + and CD4 + CD25 + cells. Quantitative real-time PCR analyses were implemented to determine the level of expression of FOXP3 and IL-2Rα (CD25) genes in the peripheral blood mononucleated cells. Results We found a significant higher proportion of CD4 + T cells ( p value < 0.001), along with a significantly reduced proportion of CD4 + CD25 + Treg cells ( p value = 0.001, p value = 0.02), in the peripheral blood of patient’s with ALS. Conclusion The results of our study are in line with the hypothesis that in the early phase of ALS, neuroprotective helper T cells infiltrate in the affected areas in the lumbar spinal cord. This was reflected in higher peripheral percentage of CD4 + helper T cells and higher expression of FOXP3 and IL-2Rα. The observed demise in the number of active CD4 + CD25 + regulatory T cells might indicate early signs of progression to later stages of ALS in our study group. Interestingly, disease duration was the sole independent significant determining factor that predicted CD4+CD25+ regulatory T cell counts in the peripheral blood of patients at various stages of ALS, according to a logistic regression model.
MoreTranslated text
Key words
Amyotrophic lateral sclerosis,CD25,FOXP3,IL-2Rα,Regulatory T cell,T helper
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined