Neonates with congenital Cytomegalovirus and hearing loss identified via the universal newborn hearing screening program.

•Congenital CMV was diagnosed in 19/323 (5.9%) with confirmed hearing loss, of whom 11/323 infants had a definite diagnosis at <21 days of age.•CMV testing mirrored the timing of audiological testing.•Audiology and CMV testing was completed by 21 days for ∼40% of infants.•Audiology and CMV testing was completed by 30 days for ∼64% of infants.•No additional assets were required to those already in this paediatric centre.•This protocol allowed sensitive detection of infants with congenital CMV, although lack of testing before 21 days in some infants meant clinical specificity was potentially lowered.