Live and heat-killed Lactobacillus spp. interfere with Streptococcus mutans and Streptococcus oralis during biofilm development on titanium surface.

•Biofilm inhibition by interference studies between streptococci and lactobacilli.•Lactobacilli aggregation and cell-surface properties influence streptococci biofilm.•No differences in streptococci biofilm inhibition by live or HK lactobacilli.•CFCSs of lactobacilli strains decrease streptococci biofilm formation.•Lactobacilli show different mechanisms in streptococci biofilm inhibition.