A Culture Model to Study Neuron-Schwann Cell-Astrocyte Interactions.
SCHWANN CELLS: METHODS AND PROTOCOLS(2018)
Abstract
In vitro models using Schwann cell and astrocyte co-cultures have been used to understand the mechanisms underlying the formation of boundaries between these cells in vivo. Schwann cell/astrocyte co-cultures also mimic the in vivo scenario of a transplant in a spinal cord injury site, thereby allowing testing of therapeutic approaches. In this chapter, we describe a triple cell culture system with Schwann cells, astrocytes, and neurons that replicates axon growth from a Schwann cell graft into an astrocyte-rich region. In vitro studies using this model can accelerate the discovery of more effective therapeutic combinations to be used along with Schwann cell transplantation after spinal cord injuries.
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Key words
Astrocytes,Axon growth,Co-cultures,Glial barrier,Neurons,Schwann cells
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