Neptunicella Marina Gen. Nov., Sp Nov., Isolated From Surface Seawater

A Gram-stain-negative, aerobic, short rod-shaped bacterium with a single polar flagellum, designated strain S27-2(T), was isolated from surface seawater from the Indian Ocean. Growth was observed in 0-12.0% (w/v) NaCl with an optimum of 0.5-2.0% (w/v) NaCl, pH 6.0-9.0 with an optimum of pH 7.0, and growth temperature of 10-41 degrees C with an optimum of 25-37 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S27-2(T) belonged to the family Alteromonadaceae and formed a distinct lineage with the type strain of Pseudobowmanella zhangzhouensis. Levels of 16S rRNA gene sequence similarity between strain S27-2(T) and members of related genera included in the trees ranged from 86.7 to 93.8 %. Strain S27-2(T) contained Q-8 as the predominant ubiquinone. The principal fatty acids (> 10 %) were C-16:0 (22.1 %), C-16:1 omega 7c/omega 6c (22.7 %) and C-18:1 omega 7c/omega 6c (20.1 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and two unknown lipids. The G+C content of strain S27-2(T) was 43.7 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, strain S27-2(T) should be classified as a novel species in a new genus within the family Alteromonadaceae, for which the name Neptunicella marina gen. nov., sp. nov. is proposed, with the type strain S27-2(T) (= KCTC52335(T) = MCCC 1A02149(T)).