Heritable genetic transformation of Strongyloides stercoralis by microinjection of plasmid DNA constructs into the male germline.

•Strongyloides sperm are transformed by injecting DNA into the free-living male testes.•Transformed males transmit transgenes to progeny by mating with wild-type females.•Transgene propagation is enhanced by multiple matings of transgenic males.•Progeny of transformed males and females inherit transgenes from both parents.