MiR-144 Affects Fatty Acid Composition by Regulating ELOVL6 Expression in Duck Hepatocytes.
Cell biology international(2017)
摘要
Lipid metabolism in duck is very important for both raisers and people's health. In our previous studies, we have detected that miR-144 is related to duck lipid metabolism and validated one of its target genes, elongation of very long chain fatty acids protein 6 (ELOVL6). In the present study, we isolated, cultured, and identified duck hepatocytes, and transfected with miR-144 mimics/inhibitor to mediate the miR-144 level. The qRT-PCR results showed that the ELOVL6 expression in duck hepatocytes was down/upregulated, respectively. The fat contents and each fatty-acid percent content of the hepatocytes and medium were also determined. When ELOVL6 expression suppressed (miR-144 mimics transfected), the palmitic acid (C16:0) content was significantly increased (P < 0.05); the oleic acid (C18:1, n-9), eicosenoic acid (C20:1, n-9), and eicosatrienoic acid (C20:3) contents were significantly reduced (P < 0.05). The myristic acid (C14:0) and palmitic acid (C16:0) contents were significantly reduced (P < 0.05), and the oleic acid (C18:1, n-9) content was significantly increased (P < 0.05) when ELOVL6 expression upregulated (miR-144 inhibitor transfected). It indicated that miR-144 could regulate some saturated fatty acids elongated to longer unsaturated fatty acids through controlling ELOVL6 expression. Whereas, miR-144/ELOVL6 appeared not associated with fat deposition in duck hepatocytes (P > 0.05). Our findings suggest that miR-144 might regulate the percentages of fatty acids in duck hepatocytes through affecting ELOVL6 expression.
更多查看译文
关键词
duck,ELOVL6,hepatocyte,lipid metabolism,microRNA,miR-144
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要