Comparison of a new visual isothermal nucleic acid amplification test with PCR and skin snip analysis for diagnosis of onchocerciasis in humans.

Andy Alhassan,Mike Y Osei-Atweneboana,Kwadwo F Kyeremeh,Catherine B Poole,Zhiru Li,Edward Tettevi,Nathan A Tanner,Clotilde K S Carlow

Molecular and Biochemical Parasitology（2016）

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摘要

•Developed a simple and accurate assay to detect Onchocerca volvulus in humans.•O-150 LAMP is more sensitive than microscopic detection of microfilariae or PCR.•Colorimetric O-150 LAMP may be useful for onchocerciasis surveillance.

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关键词

LAMP,PCR,O-150

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