Improved Murine-MHC-Deficient HLA-Transgenic NOD-Mouse Models for Type 1 Diabetes Therapy Development.
DIABETES(2018)
Abstract
Improved mouse models for type 1 diabetes (T1D) therapy development are needed. T1D susceptibility is restored to normally resistant NOD.beta 2m(-/-) mice transgenically expressing human disease-associated HLA-A*02:01 or HLA-B*39:06 class I molecules in place of their murine counterparts. T1D is dependent on pathogenic CD8(+) T-cell responses mediated by these human class I variants. NOD.beta 2m(-/-) -A2.1 mice were previously used to identify beta-cell autoantigens presented by this human class I variant to pathogenic CD8(+) T cells and for testing therapies to attenuate such effectors. However, NOD. beta 2m(-/-) mice also lack nonclassical MHC I family members, including FcRn, required for antigen presentation, and maintenance of serum IgG and albumin, precluding therapies dependent on these molecules. Hence, we used CRISPR/Cas9 to directly ablate the NOD H2-K-d and H2-D-b classical class I variants either individually or in tandem (cMHCl(-/-)). Ablation of the H2-A(g7) class II variant in the latter stock created NOD mice totally lacking in classical murine MHC expression (cMHCI/II-/-). NOD-cMHCl(-/-) mice retained nonclassical MHC I molecule expression and FcRn activity. Transgenic expression of HLA-A2 or -B39 restored pathogenic CD8(+) T-cell development and T1D susceptibility to NOD-cMHCl(-/-) mice. These next-generation HLA-humanized NOD models may provide improved platforms for T1D therapy development.
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Key words
Type 1 Diabetes
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