Boronate affinity electrophoresis for the purification and analysis of cofactor-modified RNAs.

Gabriele Nübel,Frieda A Sorgenfrei,Andres Jäschke

Methods（2017）

引用 38|浏览5

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摘要

•Boronic acid-modified polyacrylamide gels retard NAD-RNA compared to unmodified RNAs.•In vitro transcribed NAD-RNA can be purified on boronic acid-modified gels.•The kinetics of NAD-RNA decapping enzymes can be easily measured.•The NAD modification status of cellular sRNAs can be determined.•Boronic acid-modified gels offer a possibility to screen for NAD-RNA in total RNA.

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关键词

RNA modification,NAD,Affinity electrophoresis,Boronic acid,NAD-RNA,FAD,FAD-RNA

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