Studying the Dynamics of TCR Internalization at the Immune Synapse.
Methods in molecular biology (Clifton, N.J.)(2017)
摘要
Establishing a stable interaction between a T cell and an antigen presenting cell (APC) involves the formation of an immune synapse (IS). It is through this structure that the T cell can integrate all the signals provided by the APC. The IS also serves as a mechanism for TCR downregulation through internalization. Here, we describe methods for visualizing MHC-engaged T cell receptor (TCR) internalization from the IS in human cell lines and mouse primary T cells by confocal fluorescence microscopy techniques.
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关键词
Antigen presenting cell,Confocal fluorescence microscopy,Immune synapse,MHC-peptide,SMAC,T cell,TCR internalization
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