Marivivens Niveibacter Sp Nov., Isolated From The Seawater Of Tropical Mangrove

A novel bacterial strain, designated HSLHS2(T), was isolated from the seawater of a tropical mangrove forest. Cells of strain HSLHS2(T) were found to be aerobic, Gram-stain-negative, non-flagellated, non-motile, short rods. Oxidase- and catalase-positive. Growth was observed at 5-40 degrees C (optimum, 35 degrees C), at pH 6.0-10.0 (optimum pH 8.0) and in 0-10% NaCl (optimum 2 %, w/v). Strain HSLHS2(T) shared highest 16S rRNA gene sequence similarity with Celeribacter halophilus ZXM137(T) (95.4 %), but formed a distinct phyletic lineage and coherent phylogenetic cluster associated with Marivivens donghaensis AM-4(T) (95.1 %). The dominant fatty acids were summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c) and C-16 : 0. The respiratory quinone was determined to be Q-10. The polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids, four unidentified lipids, five unidentified phospholipids. The DNA G+C contents was 54.6 mol%. The combined genotypic and phenotypic data indicated that strain HSLHS2(T) represents a novel species of the genus Marivivens, for which the name Marivivens niveibacter sp. nov. is proposed. The type strain is HSLHS2(T) (=KCTC 52588(T) =MCCC 1A06712(T)).