Different Dietary Proportions Of Fish Oil Regulate Inflammatory Factors But Do Not Change Intestinal Tight Junction Zo-1 Expression In Ethanol-Fed Rats

Sixty male Wistar rats were fed a control or an ethanol-containing diet in groups C or E. The fat compositions were adjusted with 25% or 57% fish oil substituted for olive oil in groups CF25, CF57, EF25, and EF57. Hepatic thiobarbituric acid-reactive substance (TBARS) levels, cytochrome P450 2E1 protein expression, and tumor necrosis factor-(TNF-) alpha, interleukin-(IL-) 1 beta, IL-6, and IL-10 levels, as well as intracellular adhesion molecule (ICAM)-1 levels were significantly elevated, whereas plasma adiponectin level was significantly reduced in group E (p < 0 05). Hepatic histopathological scores of fatty change and inflammation, in group E were significantly higher than those of group C (p < 0 05). Hepatic TBARS, plasma ICAM-1, and hepatic TNF-alpha, IL-1 beta, and IL-10 levels were significantly lower, and plasma adiponectin levels were significantly higher in groups EF25 and EF57 than those in group E (p < 0 05). The immunoreactive area of the intestinal tight junction protein, ZO-1, showed no change between groups C and E. Only group CF57 displayed a significantly higher ZO-1 immunoreactive area compared to group C (p = 0 0415). 25% or 57% fish oil substituted for dietary olive oil could prevent ethanol-induced liver damage in rats, but the mechanism might not be related to intestinal tight junction ZO-1 expression.