More Reasonable Animal Model for Study the Effect of Pneumoperitoneum on Abdominal Tumor Cells
Asian Pacific journal of cancer prevention : APJCP(2018)
Abstract
Background: Many animal experimental studies showed that abdominal tumor cells will be widely spread during
laparoscopic treatment and grow into metastases. These results are different from clinical observations. There is a
hypothesis that too much tumor cells was injected in the animals lead to the results of theses bias. We aim to learn the
difference of abdominal cavity volume between human body and the nude mice and to determine reasonable amount
of tumor cells in the animal experiments. Methods: The insufflated CO2 volume which represents the capacity of the
abdominal cavity was recorded during laparoscopic process in 212 patients and 20 nude mice respectively, the relative
volume of nude mice and human body was calculated.Based on data from the literature and this study , the amount
of tumor cells in the animal experiments was determined.According to these data, we set up a new animal model
and a traditional one respectively,and compared the rate of successful modeling and tumor formation between two
animal models. Results: The intraperitoneal volumes of humans and nude mice were 3.01±0.36 L and 0.011±0.001 L
respectively.The number of tumor cells that be uesd in animal should be approximately 0.26×105 in terms of known
data in human beings.Compared with the traditional animal model which formed a large number of intraperitoneal
tumor metastasis, the new animal model was shows more moderately,and the rate of successful modeling was similar.
Conclusion: In animal experiments, to simulate the clinical situation, about 0.26×105 tumor cells should be inject in
peritoneal cavity of the nude mice.
MoreTranslated text
Key words
Human body,pneumoperitoneum,tumor cells,animal models
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