Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses.

Jesse J Waggoner,Lionel Gresh,Alisha Mohamed-Hadley,Gabriela Ballesteros, Maria Jose Vargas Davila,Yolanda Tellez,Malaya K Sahoo,Angel Balmaseda,Eva Harris,Benjamin A Pinsky

EMERGING INFECTIOUS DISEASES（2016）

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摘要

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

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关键词

Zika virus,arboviruses,chikungunya virus,dengue virus,multiplex molecular diagnostics,real-time RT-PCR,vector-borne infections,viruses

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