Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses.

Andy Kilianski,Pierce A Roth,Alvin T Liem,Jessica M Hill,Kristen L Willis,Rebecca D Rossmaier, Andrew V Marinich,Michele N Maughan,Mark A Karavis,Jens H Kuhn,Anna N Honko,C Nicole Rosenzweig

EMERGING INFECTIOUS DISEASES（2016）

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摘要

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.

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关键词

Ebola virus,RNA virus,Venezuelan equine encephalitis virus,Zika virus,fieldable platform,genomics,nanopore sequencing,sequencing,viruses

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