Lipid Profiling of Complex Biological Mixtures by Liquid Chromatography/mass Spectrometry Using a Novel Scanning Quadrupole Data-Independent Acquisition Strategy.

RATIONALE:A novel data-independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time-of-flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches.METHODS:Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented.RESULTS:The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data.CONCLUSIONS:In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification.