Bridging the gap between comprehensive extraction protocols in plant metabolomics studies and method validation.
Analytica Chimica Acta(2016)
摘要
It is vital to pay much attention to the design of extraction methods developed for plant metabolomics, as any non-extracted or converted metabolites will greatly affect the overall quality of the metabolomics study. Method validation is however often omitted in plant metabolome studies, as the well-established methodologies for classical targeted analyses such as recovery optimization cannot be strictly applied. The aim of the present study is to thoroughly evaluate state-of-the-art comprehensive extraction protocols for plant metabolomics with liquid chromatography-photodiode array-accurate mass mass spectrometry (LC-PDA-amMS) by bridging the gap with method validation.
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关键词
Comprehensive phytochemical extraction,Plant metabolomics,Liquid-chromatography-accurate mass mass spectrometry,Validation
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