3-Hydroxy-3-methylglutaryl flavone glycosides from the leaves of Turpinia arguta.

Three new 3-hydroxy-3-methylglutaryl (HMG) flavone 7-O-diglycosides, argutosides A-C (1–3); two new flavone 7-O-triglycosides, argutosides D-E (4–5); and one known apigenin 7-O-triglycoside (6), were isolated from the leaves of Turpinia arguta. The structures of these compounds were elucidated by spectroscopic and chemical techniques. The NO inhibitory activities of compounds 1–6 were evaluated using lipopolysaccharide-induced RAW264.7 cells. Only compound 2 showed a moderate inhibitory effect on NO production with an IC50 value of 25.74μM. Compounds 1–6 were not cytotoxic to RAW264.7 cells at 10μM.