High-resolution melting (HRM) for genotyping bovine ephemeral fever virus (BEFV).

Oran Erster,Rotem Stram, Shopia Menasherow, Marisol Rubistein-Giuni,Binyamin Sharir, Evgeni Kchinich,Yehuda Stram

Virus Research（2017）

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摘要

•Genotyping of BEFV by HRM based on the GC content of the amplified fragment.•Each assay relies on the use of one pair of primers.•The test is constructed using our newly developed algorithm.•The test enables the designation of the origin of each isolates.•This algorithm can also be applicable for genotyping of other viruses or genes.

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关键词

HRM analysis,Bovine ephemeral fever virus,Genotyping,GC content

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