Lateral flow immunoassay and enzyme linked immunosorbent assay as effective immunomethods for the detection of synthetic cannabinoid JWH-200 based on the newly synthesized hapten.

•A hapten with [(morpholin-4-yl)alkyl]indole moiety is used in the detection of JWH-200.•The hapten is synthesized via Rosenmund–von Braun and Friedel-Crafts reactions.•ELISA and LFIA are developed and used for quantification of JHW-200 in saliva samples.•Our one-step LFIA provides a visual evidence of JWH-200 presence within 15 min.