Comparison of diagnostic techniques for the detection and differentiation of Cherry leaf roll virus strains for quarantine purposes.
Journal of Virological Methods(2016)
摘要
•CLRV isolated from seven plant species revealed biological differences.•One out of four commercially-available antisera successfully detected CLRV isolates.•Parts of the coat protein, polymerase and 3ʹ-untranslated regions were sequenced.•Some CLRV isolates group differently depending on the gene studied.•Conventional RT-PCR using primers targeting the coat protein region is recommended.
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关键词
Biological,Serology,Molecular,Strains,Cherry leaf roll virus,Quarantine
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