Live-cell measurements of kinase activity in single cells using translocation reporters

Takamasa Kudo,Stevan Jeknić,Derek N Macklin,Sajia Akhter,Jacob J Hughey,Sergi Regot,Markus W Covert

NATURE PROTOCOLS（2017）

引用 75|浏览13

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摘要

This protocol describes how to design and use fluorescent translocation reporters for live measurements of kinase activity in single cells.

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关键词

Image processing,Kinases,Protein translocation,Sensors and probes,Single-cell imaging,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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