Circle scanning STED fluorescence correlation spectroscopy to quantify membrane dynamics and compartmentalization.

Riccardo Maraspini,Oliver Beutel,Alf Honigmann

Methods（2018）

引用 12|浏览6

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摘要

•Mapping of cell membrane dynamics with high spatiotemporal resolution.•Combination of scanning FCS with super-resolution STED microscopy.•Spatiotemporal heterogeneity of lipid probes in living cells.•Testing for signatures of lipid phase separation in the cell membranes.•Comparison of lipid diffusion in the cell membrane of different epithelial cell lines.

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关键词

Scanning fluorescence correlation spectroscopy,Super-resolution STED microscopy,Membrane dynamics,Lipid phase separation,Sub-diffusion

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