A reduction in long-term spatial memory persists after discontinuation of peripubertal GnRH agonist treatment in sheep.

•Peripubertal GnRHa impaired long-term spatial memory.•This impairment was not reversed after discontinuing GnRHa-treatment.•Spatial orientation and learning performance remained unaffected following GnRHa withdrawal.•Speed of progression through these spatial tasks was altered after discontinuing GnRHa.•GnRH irreversibly alters these cognitive functions during critical window of development.