The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.

Pietro Marini,Maria Grazia Cascio,Roger G Pertwee

ENDOCANNABINOID SIGNALING: METHODS AND PROTOCOLS（2016）

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摘要

The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.

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关键词

3-Isobutyl-1-methylxanthine (IBMX),Agonist,Antagonist,Cannabinoid receptor type 2,Cyclic AMP,Forskolin (FSK),Inverse agonist

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