Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview.

Irene Consuegra, Clara Rodríguez-Aierbe, Inés Santiuste, Anna Bosch, Rosario Martínez-Marín, Ma Antonia Fortuto,Tatiana Díaz,Salvador Martí,Ma Ángeles Muñoz-Fernández

BIOPRESERVATION AND BIOBANKING（2017）

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摘要

The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.

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关键词

PBMCs,SOP,biobanks,cryopreservation,functionality

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