柴芩承气汤对急性胰腺炎大鼠肺泡巨噬细胞PERK基因表达的影响
Abstract
目的探讨柴芩承气汤(Chai Qin Cheng Qi decoction,CQCQD)对急性胰腺炎(acute pancreatitis,AP)大鼠肺泡巨噬细胞内质网双链RNA依赖蛋白激酶样ER激酶(PKR-like ER kinase,PERK)mRNA表达的影响。方法将30只SD大鼠随机分成对照组、AP组及CQCQD组(n=10)。AP组采用20%L-精氨酸腹腔注射(2.5g·kg-1·1h-1×2)制作;CQCQD组分别于第0、2、4h用CQCQD按0.2mL/100g灌胃,对照组采用生理盐水代替CQCQD灌胃,容积及方法与CQCQD组同。于6h收集胰腺组织行病理检查,肺泡巨噬细胞检测PERK的mRNA表达,血清检测大鼠血清IL-6及TNF-α水平。结果 AP组胰腺组织病理评分(8.8±0.5)分,高于对照组(1.0±0.3)分及CQCQD组(5.4±0.6)分(P<0.05);CQCQD组血清IL-6及TNF-α(48.3±10.7pg/mL,35.1±14.5pg/mL)高于对照组(35.1±10.6pg/mL,21.3±14.2pg/mL,P<0.05),但低于AP组血清(67.5±13.1pg/mL,52.1±13.7pg/mL,P<0.05)。与对照组比较,AP组巨噬细胞PERK mRNA表达上调,CQCQD灌胃可下调其表达(P<0.05)。结论 CQCQD减少炎性因子释放可能与下调巨噬细胞内质网PERK mRNA表达有关。
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Key words
Chai Qin Cheng Qi Decoction,PKR-like ER kinase,Acute pancreatitis,Alveolar macrophage
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