Responses of Atlantic cod head kidney leukocytes to phytase produced by gastrointestinal-derived bacteria
Fish Physiology and Biochemistry(2010)
摘要
This study identified phytase-producing bacteria that were previously isolated from the gastrointestinal tract of Atlantic cod, and determined its effect on head kidney leukocytes. Out of the 216 bacterial strains tested, the two phytase producers were identified as sp. and sp. based on their 16S rDNA sequence. Crude phytase from these two bacterial strains was produced employing the shake flask method. Even though the total protein of the crude phytase was not significantly different for the two bacteria, the phytase activity of the crude enzyme produced by sp. (97.1 ± 16.7 U) was significantly higher than that of the enzyme from sp. (75.9 ± 2.4 U). When cod head kidney leukocytes were incubated with the crude phytase (50 μg ml), it resulted in enhanced cell proliferation, higher myeloperoxidase, and acid phosphatase activities. Extracellular responses—respiratory burst activity and hydrogen peroxide production were not enhanced by the crude enzyme. As a consequence, the growth of two pathogenic bacteria and was not suppressed by the supernatants obtained from head kidney leukocytes incubated with the crude bacterial phytase. Thus, the enzyme from phytase-producing intestinal bacteria of Atlantic cod can stimulate intracellular head kidney leukocyte activities but not the production of extracellular substances that are involved in antibacterial response. These have implications on the potential use of bacterial phytase as feed supplement to boost cellular immune response of the fish and could be employed as a health management strategy in culture systems.
更多查看译文
关键词
Phytase,Cellular response,HK leukocytes,Atlantic cod,Gastrointestinal tract
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要